Two sample t test - equal variances not assumed - overview

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Two sample $t$ test - equal variances not assumed
Two way ANOVA
Two way ANOVA
Independent/grouping variableIndependent/grouping variablesIndependent/grouping variables
One categorical with 2 independent groupsTwo categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$)Two categorical, the first with $I$ independent groups and the second with $J$ independent groups ($I \geqslant 2$, $J \geqslant 2$)
Dependent variableDependent variableDependent variable
One quantitative of interval or ratio levelOne quantitative of interval or ratio levelOne quantitative of interval or ratio level
Null hypothesisNull hypothesisNull hypothesis
H0: $\mu_1 = \mu_2$

Here $\mu_1$ is the population mean for group 1, and $\mu_2$ is the population mean for group 2.
ANOVA $F$ tests:
  • H0 for main and interaction effects together (model): no main effects and interaction effect
  • H0 for independent variable A: no main effect for A
  • H0 for independent variable B: no main effect for B
  • H0 for the interaction term: no interaction effect between A and B
Like in one way ANOVA, we can also perform $t$ tests for specific contrasts and multiple comparisons. This is more advanced stuff.
ANOVA $F$ tests:
  • H0 for main and interaction effects together (model): no main effects and interaction effect
  • H0 for independent variable A: no main effect for A
  • H0 for independent variable B: no main effect for B
  • H0 for the interaction term: no interaction effect between A and B
Like in one way ANOVA, we can also perform $t$ tests for specific contrasts and multiple comparisons. This is more advanced stuff.
Alternative hypothesisAlternative hypothesisAlternative hypothesis
H1 two sided: $\mu_1 \neq \mu_2$
H1 right sided: $\mu_1 > \mu_2$
H1 left sided: $\mu_1 < \mu_2$
ANOVA $F$ tests:
  • H1 for main and interaction effects together (model): there is a main effect for A, and/or for B, and/or an interaction effect
  • H1 for independent variable A: there is a main effect for A
  • H1 for independent variable B: there is a main effect for B
  • H1 for the interaction term: there is an interaction effect between A and B
ANOVA $F$ tests:
  • H1 for main and interaction effects together (model): there is a main effect for A, and/or for B, and/or an interaction effect
  • H1 for independent variable A: there is a main effect for A
  • H1 for independent variable B: there is a main effect for B
  • H1 for the interaction term: there is an interaction effect between A and B
AssumptionsAssumptionsAssumptions
  • Within each population, the scores on the dependent variable are normally distributed
  • Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
  • Within each of the $I \times J$ populations, the scores on the dependent variable are normally distributed
  • The standard deviation of the scores on the dependent variable is the same in each of the $I \times J$ populations
  • For each of the $I \times J$ groups, the sample is an independent and simple random sample from the population defined by that group. That is, within and between groups, observations are independent of one another
  • Equal sample sizes for each group make the interpretation of the ANOVA output easier (unequal sample sizes result in overlap in the sum of squares; this is advanced stuff)
  • Within each of the $I \times J$ populations, the scores on the dependent variable are normally distributed
  • The standard deviation of the scores on the dependent variable is the same in each of the $I \times J$ populations
  • For each of the $I \times J$ groups, the sample is an independent and simple random sample from the population defined by that group. That is, within and between groups, observations are independent of one another
  • Equal sample sizes for each group make the interpretation of the ANOVA output easier (unequal sample sizes result in overlap in the sum of squares; this is advanced stuff)
Test statisticTest statisticTest statistic
$t = \dfrac{(\bar{y}_1 - \bar{y}_2) - 0}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}} = \dfrac{\bar{y}_1 - \bar{y}_2}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}}$
Here $\bar{y}_1$ is the sample mean in group 1, $\bar{y}_2$ is the sample mean in group 2, $s^2_1$ is the sample variance in group 1, $s^2_2$ is the sample variance in group 2, $n_1$ is the sample size of group 1, and $n_2$ is the sample size of group 2. The 0 represents the difference in population means according to the null hypothesis.

The denominator $\sqrt{\frac{s^2_1}{n_1} + \frac{s^2_2}{n_2}}$ is the standard error of the sampling distribution of $\bar{y}_1 - \bar{y}_2$. The $t$ value indicates how many standard errors $\bar{y}_1 - \bar{y}_2$ is removed from 0.

Note: we could just as well compute $\bar{y}_2 - \bar{y}_1$ in the numerator, but then the left sided alternative becomes $\mu_2 < \mu_1$, and the right sided alternative becomes $\mu_2 > \mu_1$.
For main and interaction effects together (model):
  • $F = \dfrac{\mbox{mean square model}}{\mbox{mean square error}}$
For independent variable A:
  • $F = \dfrac{\mbox{mean square A}}{\mbox{mean square error}}$
For independent variable B:
  • $F = \dfrac{\mbox{mean square B}}{\mbox{mean square error}}$
For the interaction term:
  • $F = \dfrac{\mbox{mean square interaction}}{\mbox{mean square error}}$
Note: mean square error is also known as mean square residual or mean square within.
For main and interaction effects together (model):
  • $F = \dfrac{\mbox{mean square model}}{\mbox{mean square error}}$
For independent variable A:
  • $F = \dfrac{\mbox{mean square A}}{\mbox{mean square error}}$
For independent variable B:
  • $F = \dfrac{\mbox{mean square B}}{\mbox{mean square error}}$
For the interaction term:
  • $F = \dfrac{\mbox{mean square interaction}}{\mbox{mean square error}}$
Note: mean square error is also known as mean square residual or mean square within.
n.a.Pooled standard deviationPooled standard deviation
-$ \begin{aligned} s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2}{N - (I \times J)}}\\ &= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\ &= \sqrt{\mbox{mean square error}} \end{aligned} $ $ \begin{aligned} s_p &= \sqrt{\dfrac{\sum\nolimits_{subjects} (\mbox{subject's score} - \mbox{its group mean})^2}{N - (I \times J)}}\\ &= \sqrt{\dfrac{\mbox{sum of squares error}}{\mbox{degrees of freedom error}}}\\ &= \sqrt{\mbox{mean square error}} \end{aligned} $
Sampling distribution of $t$ if H0 were trueSampling distribution of $F$ if H0 were trueSampling distribution of $F$ if H0 were true
Approximately the $t$ distribution with $k$ degrees of freedom, with $k$ equal to
$k = \dfrac{\Bigg(\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}\Bigg)^2}{\dfrac{1}{n_1 - 1} \Bigg(\dfrac{s^2_1}{n_1}\Bigg)^2 + \dfrac{1}{n_2 - 1} \Bigg(\dfrac{s^2_2}{n_2}\Bigg)^2}$
or
$k$ = the smaller of $n_1$ - 1 and $n_2$ - 1

First definition of $k$ is used by computer programs, second definition is often used for hand calculations.
For main and interaction effects together (model):
  • $F$ distribution with $(I - 1) + (J - 1) + (I - 1) \times (J - 1)$ (df model, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable A:
  • $F$ distribution with $I - 1$ (df A, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable B:
  • $F$ distribution with $J - 1$ (df B, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For the interaction term:
  • $F$ distribution with $(I - 1) \times (J - 1)$ (df interaction, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
Here $N$ is the total sample size.
For main and interaction effects together (model):
  • $F$ distribution with $(I - 1) + (J - 1) + (I - 1) \times (J - 1)$ (df model, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable A:
  • $F$ distribution with $I - 1$ (df A, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For independent variable B:
  • $F$ distribution with $J - 1$ (df B, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
For the interaction term:
  • $F$ distribution with $(I - 1) \times (J - 1)$ (df interaction, numerator) and $N - (I \times J)$ (df error, denominator) degrees of freedom
Here $N$ is the total sample size.
Significant?Significant?Significant?
Two sided: Right sided: Left sided:
  • Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or
  • Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$
  • Check if $F$ observed in sample is equal to or larger than critical value $F^*$ or
  • Find $p$ value corresponding to observed $F$ and check if it is equal to or smaller than $\alpha$
Approximate $C\%$ confidence interval for $\mu_1 - \mu_2$n.a.n.a.
$(\bar{y}_1 - \bar{y}_2) \pm t^* \times \sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}$
where the critical value $t^*$ is the value under the $t_{k}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20).

The confidence interval for $\mu_1 - \mu_2$ can also be used as significance test.
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n.a.Effect sizeEffect size
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  • Proportion variance explained $R^2$:
    Proportion variance of the dependent variable $y$ explained by the independent variables and the interaction effect together:
    $$ \begin{align} R^2 &= \dfrac{\mbox{sum of squares model}}{\mbox{sum of squares total}} \end{align} $$ $R^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\eta^2$:
    Proportion variance of the dependent variable $y$ explained by an independent variable or interaction effect:
    $$ \begin{align} \eta^2_A &= \dfrac{\mbox{sum of squares A}}{\mbox{sum of squares total}}\\ \\ \eta^2_B &= \dfrac{\mbox{sum of squares B}}{\mbox{sum of squares total}}\\ \\ \eta^2_{int} &= \dfrac{\mbox{sum of squares int}}{\mbox{sum of squares total}} \end{align} $$ $\eta^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\omega^2$:
    Corrects for the positive bias in $\eta^2$ and is equal to:
    $$ \begin{align} \omega^2_A &= \dfrac{\mbox{sum of squares A} - \mbox{degrees of freedom A} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_B &= \dfrac{\mbox{sum of squares B} - \mbox{degrees of freedom B} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_{int} &= \dfrac{\mbox{sum of squares int} - \mbox{degrees of freedom int} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \end{align} $$ $\omega^2$ is a better estimate of the explained variance in the population than $\eta^2$. Only for balanced designs (equal sample sizes).

  • Proportion variance explained $\eta^2_{partial}$: $$ \begin{align} \eta^2_{partial\,A} &= \frac{\mbox{sum of squares A}}{\mbox{sum of squares A} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,B} &= \frac{\mbox{sum of squares B}}{\mbox{sum of squares B} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,int} &= \frac{\mbox{sum of squares int}}{\mbox{sum of squares int} + \mbox{sum of squares error}} \end{align} $$
  • Proportion variance explained $R^2$:
    Proportion variance of the dependent variable $y$ explained by the independent variables and the interaction effect together:
    $$ \begin{align} R^2 &= \dfrac{\mbox{sum of squares model}}{\mbox{sum of squares total}} \end{align} $$ $R^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\eta^2$:
    Proportion variance of the dependent variable $y$ explained by an independent variable or interaction effect:
    $$ \begin{align} \eta^2_A &= \dfrac{\mbox{sum of squares A}}{\mbox{sum of squares total}}\\ \\ \eta^2_B &= \dfrac{\mbox{sum of squares B}}{\mbox{sum of squares total}}\\ \\ \eta^2_{int} &= \dfrac{\mbox{sum of squares int}}{\mbox{sum of squares total}} \end{align} $$ $\eta^2$ is the proportion variance explained in the sample. It is a positively biased estimate of the proportion variance explained in the population.

  • Proportion variance explained $\omega^2$:
    Corrects for the positive bias in $\eta^2$ and is equal to:
    $$ \begin{align} \omega^2_A &= \dfrac{\mbox{sum of squares A} - \mbox{degrees of freedom A} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_B &= \dfrac{\mbox{sum of squares B} - \mbox{degrees of freedom B} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \\ \omega^2_{int} &= \dfrac{\mbox{sum of squares int} - \mbox{degrees of freedom int} \times \mbox{mean square error}}{\mbox{sum of squares total} + \mbox{mean square error}}\\ \end{align} $$ $\omega^2$ is a better estimate of the explained variance in the population than $\eta^2$. Only for balanced designs (equal sample sizes).

  • Proportion variance explained $\eta^2_{partial}$: $$ \begin{align} \eta^2_{partial\,A} &= \frac{\mbox{sum of squares A}}{\mbox{sum of squares A} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,B} &= \frac{\mbox{sum of squares B}}{\mbox{sum of squares B} + \mbox{sum of squares error}}\\ \\ \eta^2_{partial\,int} &= \frac{\mbox{sum of squares int}}{\mbox{sum of squares int} + \mbox{sum of squares error}} \end{align} $$
Visual representationn.a.n.a.
Two sample t test - equal variances not assumed
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n.a.ANOVA tableANOVA table
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two way ANOVA table
two way ANOVA table
n.a.Equivalent toEquivalent to
-OLS regression with two categorical independent variables and the interaction term, transformed into $(I - 1)$ + $(J - 1)$ + $(I - 1) \times (J - 1)$ code variables.OLS regression with two categorical independent variables and the interaction term, transformed into $(I - 1)$ + $(J - 1)$ + $(I - 1) \times (J - 1)$ code variables.
Example contextExample contextExample context
Is the average mental health score different between men and women?Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender?Is the average mental health score different between people from a low, moderate, and high economic class? And is the average mental health score different between men and women? And is there an interaction effect between economic class and gender?
SPSSSPSSSPSS
Analyze > Compare Means > Independent-Samples T Test...
  • Put your dependent (quantitative) variable in the box below Test Variable(s) and your independent (grouping) variable in the box below Grouping Variable
  • Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
  • Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
  • Continue and click OK
Analyze > General Linear Model > Univariate...
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factor(s)
Analyze > General Linear Model > Univariate...
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factor(s)
JamoviJamoviJamovi
T-Tests > Independent Samples T-Test
  • Put your dependent (quantitative) variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
  • Under Tests, select Welch's
  • Under Hypothesis, select your alternative hypothesis
ANOVA > ANOVA
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factors
ANOVA > ANOVA
  • Put your dependent (quantitative) variable in the box below Dependent Variable and your two independent (grouping) variables in the box below Fixed Factors
Practice questionsPractice questionsPractice questions