# Two sample t test - equal variances not assumed - overview

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Two sample $t$ test - equal variances not assumed
Wilcoxon signed-rank test
Pearson correlation
Independent/grouping variableIndependent variableVariable 1
One categorical with 2 independent groups2 paired groupsOne quantitative of interval or ratio level
Dependent variableDependent variableVariable 2
One quantitative of interval or ratio levelOne quantitative of interval or ratio levelOne quantitative of interval or ratio level
Null hypothesisNull hypothesisNull hypothesis
H0: $\mu_1 = \mu_2$

Here $\mu_1$ is the population mean for group 1, and $\mu_2$ is the population mean for group 2.
H0: $m = 0$

Here $m$ is the population median of the difference scores. A difference score is the difference between the first score of a pair and the second score of a pair.

Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
H0: $\rho = \rho_0$

Here $\rho$ is the Pearson correlation in the population, and $\rho_0$ is the Pearson correlation in the population according to the null hypothesis (usually 0). The Pearson correlation is a measure for the strength and direction of the linear relationship between two variables of at least interval measurement level.
Alternative hypothesisAlternative hypothesisAlternative hypothesis
H1 two sided: $\mu_1 \neq \mu_2$
H1 right sided: $\mu_1 > \mu_2$
H1 left sided: $\mu_1 < \mu_2$
H1 two sided: $m \neq 0$
H1 right sided: $m > 0$
H1 left sided: $m < 0$
H1 two sided: $\rho \neq \rho_0$
H1 right sided: $\rho > \rho_0$
H1 left sided: $\rho < \rho_0$
AssumptionsAssumptionsAssumptions of test for correlation
• Within each population, the scores on the dependent variable are normally distributed
• Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
• The population distribution of the difference scores is symmetric
• Sample of difference scores is a simple random sample from the population of difference scores. That is, difference scores are independent of one another
Note: sometimes it considered sufficient for the data to be measured on an ordinal scale, rather than an interval or ratio scale. However, since the test statistic is based on ranked difference scores, we need to know whether a change in scores from, say, 6 to 7 is larger than/smaller than/equal to a change from 5 to 6. This is impossible to know for ordinal scales, since for these scales the size of the difference between values is meaningless.
• In the population, the two variables are jointly normally distributed (this covers the normality, homoscedasticity, and linearity assumptions)
• Sample of pairs is a simple random sample from the population of pairs. That is, pairs are independent of one another
Note: these assumptions are only important for the significance test and confidence interval, not for the correlation coefficient itself. The correlation coefficient just measures the strength of the linear relationship between two variables.
Test statisticTest statisticTest statistic
$t = \dfrac{(\bar{y}_1 - \bar{y}_2) - 0}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}} = \dfrac{\bar{y}_1 - \bar{y}_2}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}}$
Here $\bar{y}_1$ is the sample mean in group 1, $\bar{y}_2$ is the sample mean in group 2, $s^2_1$ is the sample variance in group 1, $s^2_2$ is the sample variance in group 2, $n_1$ is the sample size of group 1, and $n_2$ is the sample size of group 2. The 0 represents the difference in population means according to the null hypothesis.

The denominator $\sqrt{\frac{s^2_1}{n_1} + \frac{s^2_2}{n_2}}$ is the standard error of the sampling distribution of $\bar{y}_1 - \bar{y}_2$. The $t$ value indicates how many standard errors $\bar{y}_1 - \bar{y}_2$ is removed from 0.

Note: we could just as well compute $\bar{y}_2 - \bar{y}_1$ in the numerator, but then the left sided alternative becomes $\mu_2 < \mu_1$, and the right sided alternative becomes $\mu_2 > \mu_1$.
Two different types of test statistics can be used, but both will result in the same test outcome. We will denote the first option the $W_1$ statistic (also known as the $T$ statistic), and the second option the $W_2$ statistic. In order to compute each of the test statistics, follow the steps below:
1. For each subject, compute the sign of the difference score $\mbox{sign}_d = \mbox{sgn}(\mbox{score}_2 - \mbox{score}_1)$. The sign is 1 if the difference is larger than zero, -1 if the diffence is smaller than zero, and 0 if the difference is equal to zero.
2. For each subject, compute the absolute value of the difference score $|\mbox{score}_2 - \mbox{score}_1|$.
3. Exclude subjects with a difference score of zero. This leaves us with a remaining number of difference scores equal to $N_r$.
4. Assign ranks $R_d$ to the $N_r$ remaining absolute difference scores. The smallest absolute difference score corresponds to a rank score of 1, and the largest absolute difference score corresponds to a rank score of $N_r$. If there are ties, assign them the average of the ranks they occupy.
Then compute the test statistic:

• $W_1 = \sum\, R_d^{+}$
or
$W_1 = \sum\, R_d^{-}$
That is, sum all ranks corresponding to a positive difference or sum all ranks corresponding to a negative difference. Theoratically, both definitions will result in the same test outcome. However:
• tables with critical values for $W_1$ are usually based on the smaller of $\sum\, R_d^{+}$ and $\sum\, R_d^{-}$. So if you are using such a table, pick the smaller one.
• If you are using the normal approximation to find the $p$ value, it makes things most straightforward if you use $W_1 = \sum\, R_d^{+}$ (if you use $W_1 = \sum\, R_d^{-}$, the right and left sided alternative hypotheses 'flip').
• $W_2 = \sum\, \mbox{sign}_d \times R_d$
That is, for each remaining difference score, multiply the rank of the absolute difference score by the sign of the difference score, and then sum all of the products.
Test statistic for testing H0: $\rho = 0$:
• $t = \dfrac{r \times \sqrt{N - 2}}{\sqrt{1 - r^2}}$
where $r$ is the sample correlation $r = \frac{1}{N - 1} \sum_{j}\Big(\frac{x_{j} - \bar{x}}{s_x} \Big) \Big(\frac{y_{j} - \bar{y}}{s_y} \Big)$ and $N$ is the sample size
Test statistic for testing values for $\rho$ other than $\rho = 0$:
• $z = \dfrac{r_{Fisher} - \rho_{0_{Fisher}}}{\sqrt{\dfrac{1}{N - 3}}}$
• $r_{Fisher} = \dfrac{1}{2} \times \log\Bigg(\dfrac{1 + r}{1 - r} \Bigg )$, where $r$ is the sample correlation
• $\rho_{0_{Fisher}} = \dfrac{1}{2} \times \log\Bigg( \dfrac{1 + \rho_0}{1 - \rho_0} \Bigg )$, where $\rho_0$ is the population correlation according to H0
Sampling distribution of $t$ if H0 were trueSampling distribution of $W_1$ and of $W_2$ if H0 were trueSampling distribution of $t$ and of $z$ if H0 were true
Approximately the $t$ distribution with $k$ degrees of freedom, with $k$ equal to
$k = \dfrac{\Bigg(\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}\Bigg)^2}{\dfrac{1}{n_1 - 1} \Bigg(\dfrac{s^2_1}{n_1}\Bigg)^2 + \dfrac{1}{n_2 - 1} \Bigg(\dfrac{s^2_2}{n_2}\Bigg)^2}$
or
$k$ = the smaller of $n_1$ - 1 and $n_2$ - 1

First definition of $k$ is used by computer programs, second definition is often used for hand calculations.
Sampling distribution of $W_1$:
If $N_r$ is large, $W_1$ is approximately normally distributed with mean $\mu_{W_1}$ and standard deviation $\sigma_{W_1}$ if the null hypothesis were true. Here $$\mu_{W_1} = \frac{N_r(N_r + 1)}{4}$$ $$\sigma_{W_1} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{24}}$$ Hence, if $N_r$ is large, the standardized test statistic $$z = \frac{W_1 - \mu_{W_1}}{\sigma_{W_1}}$$ follows approximately the standard normal distribution if the null hypothesis were true.

Sampling distribution of $W_2$:
If $N_r$ is large, $W_2$ is approximately normally distributed with mean $0$ and standard deviation $\sigma_{W_2}$ if the null hypothesis were true. Here $$\sigma_{W_2} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{6}}$$ Hence, if $N_r$ is large, the standardized test statistic $$z = \frac{W_2}{\sigma_{W_2}}$$ follows approximately the standard normal distribution if the null hypothesis were true.

If $N_r$ is small, the exact distribution of $W_1$ or $W_2$ should be used.

Note: if ties are present in the data, the formula for the standard deviations $\sigma_{W_1}$ and $\sigma_{W_2}$ is more complicated.
Sampling distribution of $t$:
• $t$ distribution with $N - 2$ degrees of freedom
Sampling distribution of $z$:
• Approximately the standard normal distribution
Significant?Significant?Significant?
Two sided:
Right sided:
Left sided:
For large samples, the table for standard normal probabilities can be used:
Two sided:
Right sided:
Left sided:
$t$ Test two sided:
$t$ Test right sided:
$t$ Test left sided:
$z$ Test two sided:
$z$ Test right sided:
$z$ Test left sided:
Approximate $C\%$ confidence interval for $\mu_1 - \mu_2$n.a.Approximate $C$% confidence interval for $\rho$
$(\bar{y}_1 - \bar{y}_2) \pm t^* \times \sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}$
where the critical value $t^*$ is the value under the $t_{k}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20).

The confidence interval for $\mu_1 - \mu_2$ can also be used as significance test.
-First compute the approximate $C$% confidence interval for $\rho_{Fisher}$:
• $lower_{Fisher} = r_{Fisher} - z^* \times \sqrt{\dfrac{1}{N - 3}}$
• $upper_{Fisher} = r_{Fisher} + z^* \times \sqrt{\dfrac{1}{N - 3}}$
where $r_{Fisher} = \frac{1}{2} \times \log\Bigg(\dfrac{1 + r}{1 - r} \Bigg )$ and the critical value $z^*$ is the value under the normal curve with the area $C / 100$ between $-z^*$ and $z^*$ (e.g. $z^*$ = 1.96 for a 95% confidence interval).
Then transform back to get the approximate $C$% confidence interval for $\rho$:
• lower bound = $\dfrac{e^{2 \times lower_{Fisher}} - 1}{e^{2 \times lower_{Fisher}} + 1}$
• upper bound = $\dfrac{e^{2 \times upper_{Fisher}} - 1}{e^{2 \times upper_{Fisher}} + 1}$
n.a.n.a.Properties of the Pearson correlation coefficient
--
• The Pearson correlation coefficient is a measure for the linear relationship between two quantitative variables.
• The Pearson correlation coefficient squared reflects the proportion of variance explained in one variable by the other variable.
• The Pearson correlation coefficient can take on values between -1 (perfect negative relationship) and 1 (perfect positive relationship). A value of 0 means no linear relationship.
• The absolute size of the Pearson correlation coefficient is not affected by any linear transformation of the variables. However, the sign of the Pearson correlation will flip when the scores on one of the two variables are multiplied by a negative number (reversing the direction of measurement of that variable).
For example:
• the correlation between $x$ and $y$ is equivalent to the correlation between $3x + 5$ and $2y - 6$.
• the absolute value of the correlation between $x$ and $y$ is equivalent to the absolute value of the correlation between $-3x + 5$ and $2y - 6$. However, the signs of the two correlation coefficients will be in opposite directions, due to the multiplication of $x$ by $-3$.
• The Pearson correlation coefficient does not say anything about causality.
• The Pearson correlation coefficient is sensitive to outliers.
Visual representationn.a.n.a.
--
n.a.n.a.Equivalent to
--OLS regression with one independent variable:
• $b_1 = r \times \frac{s_y}{s_x}$
• Results significance test ($t$ and $p$ value) testing $H_0$: $\beta_1 = 0$ are equivalent to results significance test testing $H_0$: $\rho = 0$
Example contextExample contextExample context
Is the average mental health score different between men and women?Is the median of the differences between the mental health scores before and after an intervention different from 0?Is there a linear relationship between physical health and mental health?
SPSSSPSSSPSS
Analyze > Compare Means > Independent-Samples T Test...
• Put your dependent (quantitative) variable in the box below Test Variable(s) and your independent (grouping) variable in the box below Grouping Variable
• Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
• Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
• Continue and click OK
Analyze > Nonparametric Tests > Legacy Dialogs > 2 Related Samples...
• Put the two paired variables in the boxes below Variable 1 and Variable 2
• Under Test Type, select the Wilcoxon test
Analyze > Correlate > Bivariate...
• Put your two variables in the box below Variables
JamoviJamoviJamovi
T-Tests > Independent Samples T-Test
• Put your dependent (quantitative) variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
• Under Tests, select Welch's
• Under Hypothesis, select your alternative hypothesis
T-Tests > Paired Samples T-Test
• Put the two paired variables in the box below Paired Variables, one on the left side of the vertical line and one on the right side of the vertical line
• Under Tests, select Wilcoxon rank
• Under Hypothesis, select your alternative hypothesis
Regression > Correlation Matrix
• Put your two variables in the white box at the right
• Under Correlation Coefficients, select Pearson (selected by default)
• Under Hypothesis, select your alternative hypothesis
Practice questionsPractice questionsPractice questions