Two sample t test - equal variances not assumed - overview

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Two sample $t$ test - equal variances not assumed
Wilcoxon signed-rank test
One sample Wilcoxon signed-rank test
Independent/grouping variableIndependent variableIndependent variable
One categorical with 2 independent groups2 paired groupsNone
Dependent variableDependent variableDependent variable
One quantitative of interval or ratio levelOne quantitative of interval or ratio levelOne of ordinal level
Null hypothesisNull hypothesisNull hypothesis
H0: $\mu_1 = \mu_2$

Here $\mu_1$ is the population mean for group 1, and $\mu_2$ is the population mean for group 2.
H0: $m = 0$

Here $m$ is the population median of the difference scores. A difference score is the difference between the first score of a pair and the second score of a pair.

Several different formulations of the null hypothesis can be found in the literature, and we do not agree with all of them. Make sure you (also) learn the one that is given in your text book or by your teacher.
H0: $m = m_0$

Here $m$ is the population median, and $m_0$ is the population median according to the null hypothesis.
Alternative hypothesisAlternative hypothesisAlternative hypothesis
H1 two sided: $\mu_1 \neq \mu_2$
H1 right sided: $\mu_1 > \mu_2$
H1 left sided: $\mu_1 < \mu_2$
H1 two sided: $m \neq 0$
H1 right sided: $m > 0$
H1 left sided: $m < 0$
H1 two sided: $m \neq m_0$
H1 right sided: $m > m_0$
H1 left sided: $m < m_0$
AssumptionsAssumptionsAssumptions
  • Within each population, the scores on the dependent variable are normally distributed
  • Group 1 sample is a simple random sample (SRS) from population 1, group 2 sample is an independent SRS from population 2. That is, within and between groups, observations are independent of one another
  • The population distribution of the difference scores is symmetric
  • Sample of difference scores is a simple random sample from the population of difference scores. That is, difference scores are independent of one another
Note: sometimes it considered sufficient for the data to be measured on an ordinal scale, rather than an interval or ratio scale. However, since the test statistic is based on ranked difference scores, we need to know whether a change in scores from, say, 6 to 7 is larger than/smaller than/equal to a change from 5 to 6. This is impossible to know for ordinal scales, since for these scales the size of the difference between values is meaningless.
  • The population distribution of the scores is symmetric
  • Sample is a simple random sample from the population. That is, observations are independent of one another
Test statisticTest statisticTest statistic
$t = \dfrac{(\bar{y}_1 - \bar{y}_2) - 0}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}} = \dfrac{\bar{y}_1 - \bar{y}_2}{\sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}}$
Here $\bar{y}_1$ is the sample mean in group 1, $\bar{y}_2$ is the sample mean in group 2, $s^2_1$ is the sample variance in group 1, $s^2_2$ is the sample variance in group 2, $n_1$ is the sample size of group 1, and $n_2$ is the sample size of group 2. The 0 represents the difference in population means according to the null hypothesis.

The denominator $\sqrt{\frac{s^2_1}{n_1} + \frac{s^2_2}{n_2}}$ is the standard error of the sampling distribution of $\bar{y}_1 - \bar{y}_2$. The $t$ value indicates how many standard errors $\bar{y}_1 - \bar{y}_2$ is removed from 0.

Note: we could just as well compute $\bar{y}_2 - \bar{y}_1$ in the numerator, but then the left sided alternative becomes $\mu_2 < \mu_1$, and the right sided alternative becomes $\mu_2 > \mu_1$.
Two different types of test statistics can be used, but both will result in the same test outcome. We will denote the first option the $W_1$ statistic (also known as the $T$ statistic), and the second option the $W_2$ statistic. In order to compute each of the test statistics, follow the steps below:
  1. For each subject, compute the sign of the difference score $\mbox{sign}_d = \mbox{sgn}(\mbox{score}_2 - \mbox{score}_1)$. The sign is 1 if the difference is larger than zero, -1 if the diffence is smaller than zero, and 0 if the difference is equal to zero.
  2. For each subject, compute the absolute value of the difference score $|\mbox{score}_2 - \mbox{score}_1|$.
  3. Exclude subjects with a difference score of zero. This leaves us with a remaining number of difference scores equal to $N_r$.
  4. Assign ranks $R_d$ to the $N_r$ remaining absolute difference scores. The smallest absolute difference score corresponds to a rank score of 1, and the largest absolute difference score corresponds to a rank score of $N_r$. If there are ties, assign them the average of the ranks they occupy.
Then compute the test statistic:

  • $W_1 = \sum\, R_d^{+}$
    or
    $W_1 = \sum\, R_d^{-}$
    That is, sum all ranks corresponding to a positive difference or sum all ranks corresponding to a negative difference. Theoratically, both definitions will result in the same test outcome. However:
    • tables with critical values for $W_1$ are usually based on the smaller of $\sum\, R_d^{+}$ and $\sum\, R_d^{-}$. So if you are using such a table, pick the smaller one.
    • If you are using the normal approximation to find the $p$ value, it makes things most straightforward if you use $W_1 = \sum\, R_d^{+}$ (if you use $W_1 = \sum\, R_d^{-}$, the right and left sided alternative hypotheses 'flip').
  • $W_2 = \sum\, \mbox{sign}_d \times R_d$
    That is, for each remaining difference score, multiply the rank of the absolute difference score by the sign of the difference score, and then sum all of the products.
Two different types of test statistics can be used, but both will result in the same test outcome. We will denote the first option the $W_1$ statistic (also known as the $T$ statistic), and the second option the $W_2$ statistic. In order to compute each of the test statistics, follow the steps below:
  1. For each subject, compute the sign of the difference score $\mbox{sign}_d = \mbox{sgn}(\mbox{score} - m_0)$. The sign is 1 if the difference is larger than zero, -1 if the diffence is smaller than zero, and 0 if the difference is equal to zero.
  2. For each subject, compute the absolute value of the difference score $|\mbox{score} - m_0|$.
  3. Exclude subjects with a difference score of zero. This leaves us with a remaining number of difference scores equal to $N_r$.
  4. Assign ranks $R_d$ to the $N_r$ remaining absolute difference scores. The smallest absolute difference score corresponds to a rank score of 1, and the largest absolute difference score corresponds to a rank score of $N_r$. If there are ties, assign them the average of the ranks they occupy.
Then compute the test statistic:

  • $W_1 = \sum\, R_d^{+}$
    or
    $W_1 = \sum\, R_d^{-}$
    That is, sum all ranks corresponding to a positive difference or sum all ranks corresponding to a negative difference. Theoratically, both definitions will result in the same test outcome. However:
    • Tables with critical values for $W_1$ are usually based on the smaller of $\sum\, R_d^{+}$ and $\sum\, R_d^{-}$. So if you are using such a table, pick the smaller one.
    • If you are using the normal approximation to find the $p$ value, it makes things most straightforward if you use $W_1 = \sum\, R_d^{+}$ (if you use $W_1 = \sum\, R_d^{-}$, the right and left sided alternative hypotheses 'flip').
  • $W_2 = \sum\, \mbox{sign}_d \times R_d$
    That is, for each remaining difference score, multiply the rank of the absolute difference score by the sign of the difference score, and then sum all of the products.
Sampling distribution of $t$ if H0 were trueSampling distribution of $W_1$ and of $W_2$ if H0 were trueSampling distribution of $W_1$ and of $W_2$ if H0 were true
Approximately the $t$ distribution with $k$ degrees of freedom, with $k$ equal to
$k = \dfrac{\Bigg(\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}\Bigg)^2}{\dfrac{1}{n_1 - 1} \Bigg(\dfrac{s^2_1}{n_1}\Bigg)^2 + \dfrac{1}{n_2 - 1} \Bigg(\dfrac{s^2_2}{n_2}\Bigg)^2}$
or
$k$ = the smaller of $n_1$ - 1 and $n_2$ - 1

First definition of $k$ is used by computer programs, second definition is often used for hand calculations.
Sampling distribution of $W_1$:
If $N_r$ is large, $W_1$ is approximately normally distributed with mean $\mu_{W_1}$ and standard deviation $\sigma_{W_1}$ if the null hypothesis were true. Here $$\mu_{W_1} = \frac{N_r(N_r + 1)}{4}$$ $$\sigma_{W_1} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{24}}$$ Hence, if $N_r$ is large, the standardized test statistic $$z = \frac{W_1 - \mu_{W_1}}{\sigma_{W_1}}$$ follows approximately the standard normal distribution if the null hypothesis were true.

Sampling distribution of $W_2$:
If $N_r$ is large, $W_2$ is approximately normally distributed with mean $0$ and standard deviation $\sigma_{W_2}$ if the null hypothesis were true. Here $$\sigma_{W_2} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{6}}$$ Hence, if $N_r$ is large, the standardized test statistic $$z = \frac{W_2}{\sigma_{W_2}}$$ follows approximately the standard normal distribution if the null hypothesis were true.

If $N_r$ is small, the exact distribution of $W_1$ or $W_2$ should be used.

Note: if ties are present in the data, the formula for the standard deviations $\sigma_{W_1}$ and $\sigma_{W_2}$ is more complicated.
Sampling distribution of $W_1$:
If $N_r$ is large, $W_1$ is approximately normally distributed with mean $\mu_{W_1}$ and standard deviation $\sigma_{W_1}$ if the null hypothesis were true. Here $$\mu_{W_1} = \frac{N_r(N_r + 1)}{4}$$ $$\sigma_{W_1} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{24}}$$ Hence, if $N_r$ is large, the standardized test statistic $$z = \frac{W_1 - \mu_{W_1}}{\sigma_{W_1}}$$ follows approximately the standard normal distribution if the null hypothesis were true.

Sampling distribution of $W_2$:
If $N_r$ is large, $W_2$ is approximately normally distributed with mean $0$ and standard deviation $\sigma_{W_2}$ if the null hypothesis were true. Here $$\sigma_{W_2} = \sqrt{\frac{N_r(N_r + 1)(2N_r + 1)}{6}}$$ Hence, if $N_r$ is large, the standardized test statistic $$z = \frac{W_2}{\sigma_{W_2}}$$ follows approximately the standard normal distribution if the null hypothesis were true.

If $N_r$ is small, the exact distribution of $W_1$ or $W_2$ should be used.

Note: if ties are present in the data, the formula for the standard deviations $\sigma_{W_1}$ and $\sigma_{W_2}$ is more complicated.
Significant?Significant?Significant?
Two sided: Right sided: Left sided: For large samples, the table for standard normal probabilities can be used:
Two sided: Right sided: Left sided:
For large samples, the table for standard normal probabilities can be used:
Two sided: Right sided: Left sided:
Approximate $C\%$ confidence interval for $\mu_1 - \mu_2$n.a.n.a.
$(\bar{y}_1 - \bar{y}_2) \pm t^* \times \sqrt{\dfrac{s^2_1}{n_1} + \dfrac{s^2_2}{n_2}}$
where the critical value $t^*$ is the value under the $t_{k}$ distribution with the area $C / 100$ between $-t^*$ and $t^*$ (e.g. $t^*$ = 2.086 for a 95% confidence interval when df = 20).

The confidence interval for $\mu_1 - \mu_2$ can also be used as significance test.
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Visual representationn.a.n.a.
Two sample t test - equal variances not assumed
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Example contextExample contextExample context
Is the average mental health score different between men and women?Is the median of the differences between the mental health scores before and after an intervention different from 0?Is the median mental health score of office workers different from $m_0 = 50$?
SPSSSPSSSPSS
Analyze > Compare Means > Independent-Samples T Test...
  • Put your dependent (quantitative) variable in the box below Test Variable(s) and your independent (grouping) variable in the box below Grouping Variable
  • Click on the Define Groups... button. If you can't click on it, first click on the grouping variable so its background turns yellow
  • Fill in the value you have used to indicate your first group in the box next to Group 1, and the value you have used to indicate your second group in the box next to Group 2
  • Continue and click OK
Analyze > Nonparametric Tests > Legacy Dialogs > 2 Related Samples...
  • Put the two paired variables in the boxes below Variable 1 and Variable 2
  • Under Test Type, select the Wilcoxon test
Specify the measurement level of your variable on the Variable View tab, in the column named Measure. Then go to:

Analyze > Nonparametric Tests > One Sample...
  • On the Objective tab, choose Customize Analysis
  • On the Fields tab, specify the variable for which you want to compute the Wilcoxon signed-rank test
  • On the Settings tab, choose Customize tests and check the box for 'Compare median to hypothesized (Wilcoxon signed-rank test)'. Fill in your $m_0$ in the box next to Hypothesized median
  • Click Run
  • Double click on the output table to see the full results
JamoviJamoviJamovi
T-Tests > Independent Samples T-Test
  • Put your dependent (quantitative) variable in the box below Dependent Variables and your independent (grouping) variable in the box below Grouping Variable
  • Under Tests, select Welch's
  • Under Hypothesis, select your alternative hypothesis
T-Tests > Paired Samples T-Test
  • Put the two paired variables in the box below Paired Variables, one on the left side of the vertical line and one on the right side of the vertical line
  • Under Tests, select Wilcoxon rank
  • Under Hypothesis, select your alternative hypothesis
T-Tests > One Sample T-Test
  • Put your variable in the box below Dependent Variables
  • Under Tests, select Wilcoxon rank
  • Under Hypothesis, fill in the value for $m_0$ in the box next to Test Value, and select your alternative hypothesis
Practice questionsPractice questionsPractice questions